Coding

Part:BBa_K654061

Designed by: Cody Tramp   Group: iGEM11_Utah_State   (2011-09-26)

Wax Ester Synthase with 8-His Tag

Maqu_0168 Wax Ester Synthase from Marinobacter aquaeolei (EC 2.3.1.75). Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction. The Wax Ester Synthase converts fatty acyl-CoA and fatty alcohols into wax esters.


2011 Utah State WaxEsterSynthetase.png


Wax Ester Synthase is used to produce Wax Esters, a valuable bioproduct, in the 2011 Utah_State iGEM project.


2011 Utah State Pathway 2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 657
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 973
    Illegal NgoMIV site found at 1080
    Illegal NgoMIV site found at 1366
    Illegal AgeI site found at 715
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None